Encyclopedia of bioinformatics and computational biology 2019.
High content screening review.
High content screening high content analysis chemdiv s hca hcs platform phenotypic assays high content screening hcs also known as high content analysis hca is a method that is used in drug discovery to identify substances that alter the phenotype of a cell in a desired manner.
Various hcs experimental applications have been utilized in the research field of in vitro toxicology.
High content imaging screening using high relevance models phenotypic hcs approaches are still largely limited to 2d cell culture systems.
Image based high content screening hcs is a high throughput screening hts technology that combines automated fluorescence microscopy from microtiter plates with digital image analysis.
High content screening hcs also known as high content analysis hca is a high throughput technique used in drug discovery to identify substances that alter the phenotype of cells.
Microscopy based high content screening image based screening is used to measure a variety of phenotypes in cells and whole organisms.
High throughput screening hts is a high tech way to hasten the drug discovery process allowing quick and efficient screening of large compound libraries at a rate of a few thousand compounds per day or per week.
This effectively allows phenotypic screening with sufficient throughput to interrogate large compound libraries.
Our high content screening portfolio consists of thermo scientific cellinsight cx5 cx7 led and cx7 lzr hcs platforms and hcs studio software designed to provide you cutting edge images and data in no time.
High content screening hcs technology combining automated microscopy and quantitative image analysis can address biological questions in academia and the pharmaceutical industry.
The microscopic images and the data derived from them provide detailed and quantitative phenotypic information about the cells in each well.
High content screening analysis hcs was invented by and registered as a trademark of cellomics which is now part of thermo fisher scientific.
2d monolayers have certainly their benefits to unravel specific biological processes but recent experience in drug discovery has shown that cells cultured as a monolayer often respond differently to drugs compared with native tissues 17.
Combined with perturbations such as rna interference small molecules and mutations such screens are a powerful method for gaining systematic insights into biological processes.
In this review.
High content screening hcs technology combining automated microscopy and quantitative image analysis can address biological questions in academia and the pharmaceutical industry.
Various hcs experimental applications have been utilized in the research field of in vitro toxicology.
Discover the power of high content screening.
